Comparative Survey of Geometric Morphometrics and Traditional Methods in Male and Female of Anjak, Schizocypris altidorsalis (Bianco and Banarescu, 1982) from Sistan Basin, Iran

Ganjali, Zohreh

doi:10.22034/cas.2023.410928.1032

Comparative Survey of Geometric Morphometrics and Traditional Methods in Male and Female of Anjak, Schizocypris altidorsalis (Bianco and Banarescu, 1982) from Sistan Basin, Iran

Document Type : Original Article

Author

Zohreh Ganjali

Department of Biology, Faculty of Basic Science, University of Zabol, Zabol, Iran

https://doi.org/10.22034/cas.2023.410928.1032

Abstract

Morphological variation between male and female populations of Anjak, Schizocypris altidorsalis species in Sistan basin of Iran was studied using landmark-based geometric morphometric and Traditional morphometric. A total of 27 specimens (16 males and 13 females) were caught from one basin. A total number of 16 landmark-points were defined and digitized on 2D pictures, and 29 morphometric characters were measured. To eliminate non-shape data, including size, direction, and position, the extracted data were analyzed by the generalized procrustes analysis. The data of TM were analyzed by Hoteling t-test, and data of GM were analyzed and compared using multivariate principal component analysis (PCA) which showed male and female populations overlapped. Deformation shape grid and Wireframe based on the consensus body shape, very little separation the two populations based on the head size, body depth, and caudal peduncle length characteristics. Based on TM analysis, these characters could not differentiate between the female and male, except for two characters, length of pectoral fin and maximum body depth (p <0.00). Comparative morphological studies revealed deformation of the grid and wireframe in more details. In the deformation grid, there are differences between the two populations due to folds and twists. Wireframe shows differences better in certain places, such as the head, caudal peduncle, and middle area of the body.

Graphical Abstract